Validation that human microbiome phages use alternative genetic coding with TAG stop read as Q
Peters, S.; Borges, A. L.; Giannone, R. J.; Morowitz, M.; Banfield, J.; Hettich, R.
Show abstract
Metagenomic findings suggesting that bacteriophages (phages) can use genetic codes different from those of their host bacteria reveal a new dimension of phage-host interaction dynamics. Whereas reassignment of stop codons to code for amino acids has been predicted, there has been no proteomic validation of alternative coding in phages. In fact, one code where the stop codon TAG is reassigned to glutamine (code 15) has never been experimentally validated in any biological system. Here, we characterized stop codon reassignment in two crAss-like phages found in the human gut microbiome using LC-MS/MS-based metaproteomics. The proteome data from several phage structural proteins clearly demonstrates reassignment of the TAG stop codon to glutamine, establishing for the first time the expression of genetic code 15. One-Sentence SummaryMass spectrometry confirms protein expression of predicted alternate genetic coding in phage genomes from human microbiomes.
Matching journals
The top 2 journals account for 50% of the predicted probability mass.
Similar papers in this journal
- Proteomic and transcriptomic analysis of Microviridae {varphi}X174 infection reveals broad up-regulation of host membrane damage and heat shock responses 95%
- Metabolome-informed microbiome analysis refines metadata classifications and reveals unexpected medication transfer in captive cheetahs 93%
- Mass spectrometry imaging of natural carbonyl products directly from agar-based microbial interactions using 4-APEBA derivatization 93%
Similar papers in this journal
Similar papers in this journal
- Large Quantities of Bacterial DNA and Protein in Common Dietary Protein Source Used in Microbiome Studies 93%
- Data-Independent Acquisition Mass Spectrometry as a Tool for Metaproteomics: Interlaboratory Comparison Using a Model Microbiome 92%
- An economic and robust TMT labeling approach for high throughput proteomic and metaproteomic analysis 91%
Similar papers in this journal
- High Resolution Imaging Mass Spectrometry of Bacterial Microcolonies at Ecological Scales 94%
- MetaX: A peptide centric metaproteomic data analysis platform using Operational Taxa-Functions (OTF) 93%
- Exploring the microbiome-wide lysine acetylation, succinylation and propionylation in human gut microbiota 93%
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.