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Mining of thousands of prokaryotic genomes reveals high abundance of prophage signals

Lopez-Leal, G.; Camelo-Valera, L. C.; Hurtado-Ramirez, J. M.; Verleyen, J.; Castillo-Ramirez, S.; Reyes-Munoz, A.

2021-10-22 bioinformatics
10.1101/2021.10.20.465230 bioRxiv
Show abstract

Phages and prophages are one of the principal modulators of microbial populations. However, much of their diversity is still poorly understood. Here, we extracted 33,624 prophages from 13,713 complete prokaryotic genomes in order to explore the prophage diversity and their relationships with their host. Our results reveal that prophages were present in 75% of the genomes studied. In addition, Enterobacterales were significantly enriched in prophages. We also found that pathogens are a significant reservoir of prophages. Finally, we determined that the prophage relatedness and the range of genomic hosts were delimited by the evolutionary relationships of their hosts. On a broader level, we got insights into the prophage population, identified in thousands of publicly available prokaryotic genomes, by comparing the prophage distribution and relatedness between them and their hosts.

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