Natural influent bioaugmentation of activated sludge water resource recovery systems: implications for low temperature nitrification and heterotrophic population structures

This work aimed at demonstrating the natural bioaugmentation of biological activated sludge systems with nitrifying biomass from influent wastewater in lab-scale reactors. Three sequencing batch reactors (SBR) were fed with sterile synthetic wastewater. While nitrification was complete at a temperature of 8 {degrees}C and a SRT of 20 days, it failed when the temperature was lowered to 5 {degrees}C, and the SRT decreased to 7 days. In the test period, the sterile synthetic wastewater fed to the Test Reactor was supplemented by influent solids harvested at a full-scale treatment facility at a total suspended solids concentration of 100 mg/L, which corresponded to approximately 5 mg-COD/L of nitrifying biomass. Upon this addition, nitrification was restores. Subsequent halting the supply of influent solids to the Test Reactor led a rapid failure of nitrification and washout of nitrifiers from the SBR. Reproducibility was demonstrated by switching the feed composition between the Test and Negative control reactors. PCR-based amplicon sequencing analyses targeting the amoA, and nxrB genes of the Nitrospira genus have shown that the influent wastewater governed the structure and composition of the activated sludge nitrifying populations. The most abundant ammonia-oxidizing bacteria (AOB) and Nitrospira-related nitrite-oxidizing bacteria (NOB) in the influent seeds occurred as the most dominant ones in the activated sludge. This pattern was observed even when the influent seeds varied over time. The heterotrophic populations were less affected by the influent seeds with the activated sludge and raw sewage showing distinct microbial populations based on principal coordinate analysis (PCoA). However, the immigrant populations appeared to modulate the structure of the activated sludge heterotrophic communities to some extent. These findings provide concrete evidence of the presence of active nitrifiers in raw wastewater capable of supporting nitrification in an otherwise non-conducive environment. This may have important implications on process design, operation and optimization of wastewater treatment systems. HighlightsO_LILab-scale reactors fed sterile synthetic wastewater at low temperatures and SRTs. C_LIO_LINitrification failed when conditions were adjusted to 5 {degrees}C and a SRT of 7 days. C_LIO_LINitrification restored by addition of real wastewater influent solids. C_LIO_LINitrifiers in solids from sewers naturally bioaugment activated sludge systems. C_LIO_LIActivated sludge models should consider the immigration of nitrifiers with influent. C_LI Graphical abstract O_FIG O_LINKSMALLFIG WIDTH=200 HEIGHT=165 SRC="FIGDIR/small/462950v1_ufig1.gif" ALT="Figure 1"> View larger version (33K): org.highwire.dtl.DTLVardef@102075dorg.highwire.dtl.DTLVardef@31aac9org.highwire.dtl.DTLVardef@c37a6dorg.highwire.dtl.DTLVardef@19aa110_HPS_FORMAT_FIGEXP M_FIG C_FIG