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Decoding microbiome and protein family linkage to improve protein structure prediction

Yang, P.; Zheng, W.; Ning, K.; Zhang, Y.

2021-04-16 bioinformatics
10.1101/2021.04.15.440088 bioRxiv
Show abstract

Information extracted from microbiome sequences through deep-learning techniques can significantly improve protein structure and function modeling. However, the model training and metagenome search were largely blind with low efficiency. Built on 4.25 billion microbiome sequences from four major biomes (Gut, Lake, Soil and Fermentor), we proposed a MetaSource model to decode the inherent link of microbial niches with protein homologous families. Large-scale protein family folding experiments showed that a targeted approach using predicted biomes significantly outperform combined metagenome datasets in both speed of MSA collection and accuracy of deep-learning structure assembly. These results revealed the important link of biomes with protein families and provided a useful bluebook to guide future microbiome sequence database and modeling development for protein structure and function prediction.

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