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A Framework for Evaluating Myocardial Stiffness Using 3D-Printed Hearts

Kolawole, F. O.; Peirlinck, M.; Cork, T. E.; Wang, V. Y.; Dual, S. S.; Levenston, M. O.; Kuhl, E.; Ennis, D. B.

2021-02-22 bioengineering
10.1101/2021.02.21.432169 bioRxiv
Show abstract

MRI-driven computational modeling is increasingly used to simulate in vivo cardiac mechanical behavior and estimate subject-specific myocardial stiffness. However, in vivo validation of these estimates is exceedingly difficult due to the lack of a known ground-truth in vivo myocardial stiffness. We have developed 3D-printed heart phantoms of known myocardium-mimicking stiffness and MRI relaxation properties and incorporated the heart phantoms within a highly controlled MRI-compatible setup to simulate in vivo diastolic filling. The setup enables the acquisition of experimental data needed to evaluate myocardial stiffness using computational constitutive modeling: phantom geometry, loading pressures, boundary conditions, and filling strains. The pressure-volume relationship obtained from the phantom setup was used to calibrate an in silico model of the heart phantom undergoing simulated diastolic filling. The model estimated stiffness was compared with ground-truth stiffness obtained from uniaxial tensile testing. Ultimately, the setup is designed to enable extensive validation of MRI and FEM-based myocardial stiffness estimation frameworks.

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