The lncRNA EPB41L4A-AS1 regulates gene expression in the nucleus and exerts cell type-dependent effects on cell cycle progression
Samdal, H.; Hegre, S. A.; Chawla, K.; Liabakk, N.-B.; Aas, P. A.; Sporsheim, B.; Saetrom, P.
Show abstract
The long non-coding RNA (lncRNA) EPB41L4A-AS1 is aberrantly expressed in various cancers and has been reported to be involved in metabolic reprogramming and as a repressor of the Warburg effect. Although the biological relevance of EPB41L4A-AS1 is evident, its functional role seems to vary depending on cell type and state of disease. By combining RNA sequencing and ChIP sequencing of cell cycle synchronized HaCaT cells we previously identified EPB41L4A-AS1 to be one of 59 lncRNAs with potential cell cycle functions. Here, we demonstrate that EPB41L4A-AS1 exists as bright foci and regulates gene expression in the nucleus in both cis and trans. Specifically, we find that EPB41L4A-AS1 positively regulates its sense overlapping gene EPB41L4A and influences expression of hundreds of other genes, including genes involved in cell proliferation. Finally, we show that EPB41L4A-AS1 affects cell cycle phase distribution, though these effects vary between cell types.
Matching journals
The top 9 journals account for 50% of the predicted probability mass.
Similar papers in this journal
Similar papers in this journal
- Loss of Lamin A leads to the nuclear translocation of AGO2 and compromised RNA interference 95%
- CRISPR-based dissection of miRNA binding sites using isogenic cell lines is hampered by pervasive noise. 94%
- A system of reporters for comparative investigation of EJC-independent and EJC-enhanced nonsense-mediated mRNA decay 94%
Similar papers in this journal
- DIS3L, cytoplasmic exosome catalytic subunit, is essential for development but not cell viability in mice. 93%
- Global Analysis by LC-MS/MS of N6-Methyladenosine and Inosine in mRNA Reveals Complex Incidence 93%
- Live-cell imaging of circular and long non-coding RNAs associated to FUS pathological aggregates by Pepper fluorescent RNA 93%
Similar papers in this journal
- APOBEC3B reporter myeloma cell lines identify DNA damage response pathways leading to APOBEC3B expression 95%
- Deciphering the role of the lncRNA TRIBAL in hepatocyte models 94%
- Single cell RNA sequencing of nc886, a non-coding RNA transcribed by RNA polymerase III, with a primer spike-in strategy 94%
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.