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Human embryonic stem cell-derived cardiomyocytes express SARS-CoV-2 host entry proteins: screen to identify inhibitors of infection

Williams, T. L.; Colzani, M. T.; Macrae, R. G.; Robinson, E. L.; Bloor, S.; Greenwood, E. J.; Zhan, J. R.; Strachan, G.; Kuc, R. E.; Nyimanu, D.; Maguire, J. J.; Lehner, P. J.; Sinha, S.; Davenport, A. P.

2021-01-22 pharmacology and toxicology
10.1101/2021.01.22.427737 bioRxiv
Show abstract

Patients with cardiovascular comorbidities are more susceptible to severe infection with SARS-CoV-2, known to directly cause pathological damage to cardiovascular tissue. We outline a screening platform using human embryonic stem cell-derived cardiomyocytes, confirmed to express the protein machinery critical for SARS-CoV-2 infection, and a pseudotyped virus system. The method has allowed us to identify benztropine and DX600 as novel inhibitors of SARS-CoV-2 infection.

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