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Two-photon microscopy at >500 volumes/second

Tsai, Y.-H.; Liu, C.-W.; Lin, W.-K.; Wang, C.-S.; Chiang, C.-H.; Singh, V. R.; So, P. T. C.; Chou, C.-F.; Chu, S.-W.

2020-10-22 biophysics
10.1101/2020.10.21.349712 bioRxiv
Show abstract

We demonstrate a multi-focal multi-photon volumetric microscopy via combination of 32-beam parallel lateral-scanning, a 70-kHz axial-scanning acoustic lens, and a 32-channel photodetector, enabling unprecedented data rate (2-10 GHz) and >500-volumes/second imaging speed over ~200x200x200-m3.

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