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Noninvasive prenatal exome sequencing inefficient for detecting single-gene disorders -- problems and possible solutions

Filer, D.; Mieczkowski, P. A.; Brandt, A.; Gilmore, K. L.; Powell, B. C.; Berg, J. S.; Wilhelmsen, K. C.; Vora, N. L.

2020-08-07 genetic and genomic medicine
10.1101/2020.08.04.20168278 medRxiv
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Whats already known about this topic?O_LISequencing-based noninvasive testing can detect large copy number abnormalities and some auto-somal dominant single-gene disorders C_LIO_LIExome sequencing (ES) on fetal samples provides 20% diagnostic yield for structural abnormalities after normal karyotype & microarray C_LI What does this study add?O_LIES on cell-free DNA in three gravid patients with suspected genetic disease in the fetus C_LIO_LIWe demonstrate broad sequencing approaches are limited by sampling and technical difficulties, concluding broad sequencing is currently inappropriate for noninvasive testing C_LI

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