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Analytical and Clinical Validation for RT-qPCR detection of SARS-CoV-2 without RNA extraction
2020-06-26
infectious diseases
Title + abstract only
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BackgroundThe recent COVID-19 pandemic has posed an unprecedented challenge to laboratory diagnosis, based on the amplification of SARS-CoV-2 RNA. With global contagion figures exceeding 4 million persons, the shortage of reagents for RNA extraction represents a bottleneck for testing globally. We present the validation results for a RT-qPCR protocol without prior RNA extraction. Because of its simplicity, this protocol is suitable for widespread application in resource-limited settings. Method...
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