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Population structure of the invasive golden mussel (Limnoperna fortunei) on reservoirs from five Brazilian drainage basins

Rodinho Nunes Ferreira, J. G.; Soares Souza, G. B.; Americo, J. A.; Silva Romao Dumaresq, A.; de Freitas Rebelo, M.

2020-05-31 genetics
10.1101/2020.05.28.122069 bioRxiv
Show abstract

The golden mussel (Limnoperna fortunei) is a freshwater bivalve that was introduced in South America almost 30 years ago, likely through ballast water of Asian ships. Since then, it has spread across the continent, causing both economic and environmental impacts. The study of the population structure of an invasive species may bring valuable insights towards understanding its pattern of dispersion, which in turn will help to create more effective management plans. Here, we have compared mussel populations from 5 different Brazilian reservoirs and tested for the presence of geographic genetic structure. In order to obtain a high number of single nucleotide variants (SNVs) at good cost-benefit, we have for the first time applied the double digest restriction-site associated DNA sequencing (ddRAD-seq) protocol for the golden mussel. The ddRAD-seq protocol allowed us to obtain 2046 SNVs, which were then used to assess population structure by applying three independent methodologies: Principal Component Analysis (PCA), Bayesian Clustering and Phylogenetic Tree. All methodologies have indicated absent geographic structure.

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