Nicotinamide mononucleotide redox cofactor system enables aldehyde accumulation in Escherichia coli
Richardson, K. N.; Black, W. B.; Li, H.
Show abstract
It is challenging to biosynthesize industrially important aldehydes, which are readily consumed by the numerous alcohol dehydrogenases (ADHs) in cells. In this work, we demonstrate that a nicotinamide mononucleotide (NMN+)-dependent redox cofactor cycling system enables aldehyde accumulation in Escherichia coli crude lysates and whole cells. By specifically delivering reducing power to a recombinant enoate reductase, but not to endogenous ADHs, we convert citral to citronellal with minimal byproduct formation (98% and 83% product purity in crude lysate- and whole cell-based biotransformation, respectively). We envision the systems universal application to lower the noise in biomanufacturing by silencing the hosts metabolic background.
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