Serpins: Purification and characterization of potent protease inhibitors from Clostridium thermocellum
Mushtaq, M.; Asad, M. J.; Hyder, M. Z.; Naqvi, S. M. S.; Malik, S. I.; Mehmood, R. T.
Show abstract
Clostridium thermocellum produces an extracellular cellulosome (a multiprotein complex produced by firmicutes bacteria), which, owing to its extracellular location, is open to protease attack. Serine protease inhibitors (serpins) protect bacteria against protease attack. However, their structure and function are poorly characterized. This study identified and amplified the serpin 1270 gene from the C. thermocellum genome. Purified serpins were cloned into the pTXB1 vector using the one-step sequence and ligation-independent cloning reaction and transformed into Escherichia coli BL21 DE3 cells. Enzyme overexpression and purification and enzyme inhibitory assays were performed. The results showed that serpin 1270 has 89% inhibition against Bacillus subtilisin and 64% inhibition against trypsin, chymotrypsin, and papain.
Matching journals
The top 5 journals account for 50% of the predicted probability mass.
Similar papers in this journal
- High-yield Production of Recombinant Platelet Factor 4 Protein by Harnessing and Honing the Gram-negative Bacterial Secretory Apparatus 96%
- Metabolic engineering of Bacillus subtilis with an Endopolygalacturonase gene Isolated from Pectobacterium carotovorum; a Plant Pathogenic bacterial strain. 95%
- Characterization of the Corynebacterium glutamicum dehydroshikimate dehydratase QsuB and its potential for microbial production of protocatechuic acid 95%
Similar papers in this journal
- Butyryl/Caproyl-CoA:Acetate CoA-Transferase: Cloning, Expression and Characterization of the Key Enzyme Involved in Medium-Chain Fatty Acid Biosynthesis 95%
- SufB intein splicing in Mycobacterium tuberculosis is influenced by two remote conserved N-extein Histidines 91%
- Identification of PCNA interacting protein motifs in human DNA polymerase delta 91%
Similar papers in this journal
- Kazal-type serine protease inhibitors from Arabidopsis thaliana and Toxoplasma gondii exhibit antimicrobial activity against plant pathogens 95%
- Characterization and description of Faecalibacterium butyricigenerans sp. nov. and F. longum sp. nov., isolated from human faeces 94%
- In vitro and in vivo efficacy of the combination of colistin and endolysins against clinical strains of Multi-Drug Resistant (MDR) pathogens 93%
Similar papers in this journal
- A novel thermostable aspartic protease from Talaromyces leycettanus and its specific autocatalytic activation through an intermediate transition state 95%
- Production and secretion of recombinant proteins using the endotoxin-free, Gram-negative bacterium Sphingobium japonicum 94%
- Identification and Evaluation of a Panel of Strong Constitutive Promoters in Listeria monocytogenes for Improving the Expression of Foreign Antigens 94%
Similar papers in this journal
- Recombinant protein production and purification of SiiD, SiiE and SiiF - components of the SPI4-encoded type I secretion system from Salmonella Typhimurium 94%
- Reliable method for high quality His-tagged and untagged E. coli phosphoribosyl phosphate synthase (Prs) purification 94%
- Expression of a human Gb3/CD77 synthase in insect and human cells: comparison of activity and glycosylation 93%
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.