Back

CRISPR-mediated biocontainment

Castanon, O.; Smith, C. J.; Khoshakhlagh, P.; Ferreira, R.; Guell, M.; Said, K.; Yildiz, R.; Dysart, M.; Wang, S.; Thompson, D.; Myllykallio, H.; Church, G. M.

2020-02-04 synthetic biology
10.1101/2020.02.03.922146 bioRxiv
Show abstract

We have exploited the repetitive nature of transposable elements of the human genome to generate synthetic circuits. Transposable elements such as LINE-1 and Alu have successfully replicated in mammalian genomes throughout evolution to reach a copy number ranging from thousands to more than a million. Targeting these repetitive elements with programmable DNA nucleases such as CRISPR-Cas9 rapidly induce extremely high levels of cell death. We use this genotoxic feature to build synthetic biocontainment circuits: CRISPR defense system (CRISPR-DS) capable of preventing CRISPR genome editing, and we introduce the proof-of-concept of CRISPR Safety-Switch, an inducible, stringent and non-leaky kill-switch capable of clearing out cell lines resistant to DNA breaks.

Matching journals

The top 1 journal accounts for 50% of the predicted probability mass.

1
The CRISPR Journal
○ 39 papers in training set
Top 0.1%
60.5%
50% of probability mass above
2
Nature Communications
○ 5641 papers in training set
Top 13%
13.1%
3
ACS Synthetic Biology
● 287 papers in training set
Top 0.6%
5.6%
4
Molecular Therapy - Nucleic Acids
○ 25 papers in training set
Top 0.1%
4.4%
5
Nucleic Acids Research
◐ 1281 papers in training set
Top 7%
2.4%
6
Scientific Reports
○ 3612 papers in training set
Top 46%
2.2%
7
Cell Death & Differentiation
○ 48 papers in training set
Top 0.9%
1.1%
8
iScience
○ 1154 papers in training set
Top 29%
1.1%
9
Nature Biotechnology
○ 172 papers in training set
Top 4%
0.9%
10
Molecular Therapy Nucleic Acids
○ 39 papers in training set
Top 0.9%
0.9%
11
Molecular Therapy
○ 81 papers in training set
Top 2%
0.6%