Characterization of novel Actinobacteriophage Giantsbane reveals potential genomic recombination hotspot and unexpected intra-cluster similarities
Liu, C.; Chen, P. Y.; Dang, P.; Zhang, M.; Kapinos, A.; Ngo, R. T.; Moberg Parker, J.; Freise, A. C.
Show abstract
Bacteriophages that infect Arthrobacter, a genus of bacteria which play key ecological roles in soil, warrant further study. Giantsbane, a novel Actinobacteriophage, was isolated using Arthrobacter globiformis as a host. Transmission electron microscopy and whole-genome sequencing revealed a Siphoviridae morphology and a genome length of 56,734 bp. Genome annotation identified 94 putative genes, such as a duplicated major tail protein and a major capsid and protease fusion protein. No genes were associated with lysogeny, indicating a lytic phage. Giantsbane was assigned to the phage cluster AU. Batch average nucleotide identity analysis and phylogenetic networks constructed from shared genes revealed unexpected nucleotide and gene content similarities within cluster AU. These findings have resulted in the creation of two new AU subclusters and the resubclustering of three AU bacteriophages. Analysis using Phamerator and MEME identified repeated motifs and a gene cassette present in all evaluated cluster AU phages which may promote recombination. These findings offer the first intra-cluster analysis of cluster AU phages and further our understanding of the relationships between closely related bacteriophages.
Matching journals
The top 10 journals account for 50% of the predicted probability mass.
Similar papers in this journal
- Isolation and Characterization of a Roseophage Representing a Novel Genus in the N4-like Rhodovirinae Subfamily Distributed in Estuarine Waters 96%
- Complete genome sequence and annotation of the laboratory reference strain Shigella flexneri serovar 5a M90T and genome-wide transcriptional start site determination 95%
- Analyses of Xenorhabdus griffiniae genomes reveal two distinct sub-species that display intra-species variation due to prophages. 95%
Similar papers in this journal
- Genomic diversity of bacteriophages infecting Rhodobacter capsulatus and their relatedness to its gene transfer agent RcGTA 96%
- Atypical integrative element with strand-biased circularization activity assists interspecies antimicrobial resistance gene transfer from Vibrio alfacsensis 95%
- Comparative evaluation of bioinformatic tools for virus-host prediction and their application to a highly diverse community in the Cuatro Cienegas Basin, Mexico 95%
Similar papers in this journal
- Genome sequence and characterization of five bacteriophages infecting Streptomyces coelicolor and Streptomyces venezuelae: Alderaan, Coruscant, Dagobah, Endor1 and Endor2 98%
- Exploring the remarkable diversity of Escherichia coli Phages in the Danish Wastewater Environment, Including 91 Novel Phage Species 96%
- Genome sequencing of the bacteriophage CL31 and interaction with the host strain Corynebacterium glutamicum ATCC 13032 96%
Similar papers in this journal
- Whole Genome Sequencing and Assembly of the House Sparrow, Passer domesticus 93%
- Chromosome-level genome assembly and annotation of the crested gecko, Correlophus ciliatus, a lizard incapable of tail regeneration 92%
- The Crown Pearl V2: an improved genome assembly of the European freshwater pearl mussel Margaritifera margaritifera (Linnaeus, 1758) 92%
Similar papers in this journal
- Revisiting typing systems for group B Streptococcus (GBS) prophages: an application in prophage detection and classification in GBS isolates from Argentina 95%
- Host interactions of novel Crassvirales species belonging to multiple families infecting bacterial host, Bacteroides cellulosilyticus WH2 94%
- Prevalence and diversity of TAL effector-like proteins in fungal endosymbiotic Mycetohabitans spp. 94%
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.