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Bright and tunable far-red chemigenetic indicators

Deo, C.; Abdelfattah, A. S.; Bhargava, H. K.; Berro, A. J.; Falco, N.; Moeyaert, B.; Chupanova, M.; Lavis, L. D.; Schreiter, E. R.

2020-01-09 molecular biology
10.1101/2020.01.08.898783 bioRxiv
Show abstract

Functional imaging using fluorescent indicators has revolutionized biology but additional sensor scaffolds are needed to access properties such as bright, far-red emission. We introduce a new platform for chemigenetic fluorescent indicators, utilizing the self-labeling HaloTag protein conjugated to environmentally sensitive synthetic fluorophores. This approach affords bright, far-red calcium and voltage sensors with highly tunable photophysical and chemical properties, which can reliably detect single action potentials in neurons.

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