On anammox activity at low temperature: effect of ladderane composition, process conditions and dominant anammox population
Kouba, V.; Hurkova, K.; Navratilova, K.; Vejmelkova, D.; Benakova, A.; Laureni, M.; Vodickova, P.; Podzimek, T.; Lipovova, P.; van Niftrik, L.; Hajslova, J.; van Loosdrecht, M. C. M.; Weissbrodt, D. G.; Bartacek, J.
Show abstract
The application of partial nitritation-anammox (PN/A) under mainstream conditions can enable substantial cost savings at wastewater treatment plants (WWTPs), but how process conditions and cell physiology affect anammox performance at psychrophilic temperatures below 15 {degrees}C remains poorly understood. We tested 14 anammox communities, including 8 from globally-installed PN/A processes, for (i) specific activity at 10-30 {degrees}C (batch assays), (ii) composition of membrane lipids (U-HPLC-HRMS/MS), and (iii) microbial community structure (16S rRNA gene amplicon sequencing). Crucially, the key parameters impacting anammox activity were the membrane lipid composition and cultivation temperature. The size of ladderane lipids and the content of bacteriohopanoids were key physiological drivers of anammox performance at low temperatures. Higher contents of (i) short C18 [3]-ladderane alkyl and (ii) large phosphatidylcholine headgroup were determined in anammox more active at 15-30 {degrees}C and 10-15 {degrees}C, respectively. At below 15 {degrees}C, the activation energies of most mesophilic cultures severely increased while those of the psychrophilic cultures remained stable; this indicates that the adaptation of mesophilic cultures to psychrophilic regime necessitates months, but in some cases can take up to 5 years. Interestingly, biomass enriched in the marine genus "Candidatus Scalindua" displayed exceptionally highest activity at 10-20 {degrees}C (0.50 kg-N.kg-VSS-1.d-1 at 10 {degrees}C, Ea10-30 {degrees}C = 51{+/-}16 kJ.mol-1), indicating outstanding potential for nitrogen removal from cold streams. Collectively, our comprehensive study provides essential knowledge of cold adaptation mechanism, will enable more accurate modelling and suggests highly promising target anammox genera for inoculation and set-up of anammox reactors, in particular for mainstream WWTPs. HighlightsO_LILadderane size and cold exposure affected anammox activation energy (Ea). C_LIO_LIEa improved with more C18 [3]-ladderanes over C20 and larger polar headgroup. C_LIO_LILong-term cold exposure reduced Ea at 10-15 {degrees}C, not activity per se. C_LIO_LIMarine "Ca. Scalindua" was exceptionally suitable for cold streams. C_LIO_LIAnammox Ea at 15-30 {degrees}C was 79{+/-}18 kJ.mol-1. C_LI Graphical abstract O_FIG O_LINKSMALLFIG WIDTH=200 HEIGHT=121 SRC="FIGDIR/small/873869v1_ufig1.gif" ALT="Figure 1"> View larger version (25K): org.highwire.dtl.DTLVardef@1c5c3aborg.highwire.dtl.DTLVardef@b11ed2org.highwire.dtl.DTLVardef@5e7ec9org.highwire.dtl.DTLVardef@1d5cba5_HPS_FORMAT_FIGEXP M_FIG C_FIG
Matching journals
The top 6 journals account for 50% of the predicted probability mass.
Similar papers in this journal
- Molecular Acclimation of Halobacterium salinarum to Halite Brine Inclusions 96%
- Isolation, identification and selection of bacteria with the proof-of-concept for bioaugmentation of whitewater from woodfree paper mills 96%
- Phototrophic co-cultures from extreme environments: community structure and potential value for fundamental and applied research 96%
Similar papers in this journal
- Adaptation of anammox bacteria to low temperature via gradual acclimation and cold shocks: distinctions in protein expression, membrane composition and activities 98%
- L-norepinephrine Induces Community Shift, Oxidative Stress Response, Metabolic Reprogramming, and Virulence Potential in Wastewater Microbiomes 97%
- Looking for lipases and lipolytic organisms in low-temperature anaerobic reactors treating domestic wastewater 97%
Similar papers in this journal
- Time series metagenomic sampling of the Thermopyles, Greece, geothermal springs reveals stable microbial communities dominated by novel sulfur-oxidizing chemoautotrophs 96%
- Short-term changes in polysaccharide utilization mechanisms of marine bacterioplankton during a spring phytoplankton bloom 95%
- Sediments from a seasonally euxinic coastal ecosystem show high nitrogen cycling potential 94%
Similar papers in this journal
- Assessing the activity of different plant-derived molecules and potential biological nitrification inhibitors on a range of soil ammonia- and nitrite- oxidizing strains 96%
- The bi-directional extracellular electron transfer process aids iron cycling by Geoalkalibacter halelectricus in a highly saline-alkaline condition 96%
- Amplicon-guided isolation and cultivation of previously uncultured microbial species from activated sludge 95%
Similar papers in this journal
- Production of nonulosonic acids in the extracellular polymeric substances of Candidatus Accumulibacter phosphatis 97%
- Synthetic and biological surfactant effects on freshwater biofilm community composition and metabolic activity 96%
- The feedstock microbiome selectively steers process stability during the anaerobic digestion of waste activated sludge 96%
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.